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06.08.2020 – 11:23

Technische Universität München

Grow faster, die sooner: How growth rates influence the fitness of bacteria

TECHNICAL UNIVERSITY OF MUNICH

Corporate Communications Center

phone: +49 89 289 10510 - e-mail: presse@tum.de - web: www.tum.de

This text on the web: https://www.tum.de/nc/en/about-tum/news/press-releases/details/36187/

High resolution images: https://mediatum.ub.tum.de/1554425

NEWS RELEASE

Grow faster, die sooner

How growth rates influence the fitness of bacteria

Bacteria are survival artists: When they get nutrition, they multiply rapidly, albeit they can also survive periods of hunger. But, when they grow too quickly, their ability to survive is hampered, as studies by a research team at the Technical University of Munich (TUM) on E. coli bacteria show. The results could help increase the effectiveness of antibiotics.

"The fitness of bacteria is more complex than expected," explains Ulrich Gerland, professor for the theory of complex biosystems at the Technical University of Munich. The physicist has been studying the survival strategies of E. coli bacteria for several years.

The unicellular organisms, which go by the Latin name Escherichia coli and support digestion in the large intestine of mammals, are a popular model organism. They facilitate investigations into the way living beings can adapt to changing environmental conditions.

Surviving hard times

"We have known for some time that biological fitness depends on two things: the growth rate when food is available and the ability to survive periods of nutrient deficiency," explains the scientist. "What was not clear is how these two factors are related."

For the first time, Gerland and his team have now systematically investigated the extent to which fast or slow growth influences the survivability of E. coli bacteria: “It turns out that changes in growth conditions have a direct impact on death rates. These follow a simple law: The best-nourished and fastest-growing bacteria are the first to die when deprived of food.”

Fat bacteria – poor fitness

So, a good diet is bad for the fitness of bacteria. But why? To find an answer to this question, the TUM researchers carried out a number of experiments: First, cultures of E. coli bacteria were provided nutrient solutions with different compositions.

In the second step, the unicellular organisms were put on a zero diet. Throughout the entire period, the scientists examined whether and how quickly the cells multiplied, and how long they survived.

The fight for survival

The research showed that regardless of how well or poorly they were previously fed, bacteria stopped reproducing when they were deprived of food. In this “maintenance phase,” organisms struggle for bare survival. All available energy sources – for example, the cellular remains of dead bacteria – are used to sustain the metabolism.

In this extreme situation, many cells die of starvation within a few days. However, the death rate is particularly high among rapidly growing E. coli bacteria. “They are primed for rapid growth and waste energy resources. This leads to their demise during the hunger phase,” explains Gerland.

As it turns out, the abundantly fed bacteria have an increased need for energy, as further experiments prove. Surviving times of scarcity is more difficult for organisms with a high energy consumption. "We now understand why evolution doesn't favor the fastest possible reproduction," says Gerland. "The biological fitness that is crucial for the survival of a species builds on a balance between growth and survivability."

Antibiotic therapy with a carrot and a stick

The research results may find application in the future, for example, to improve the effect of antibiotics: “Applying a carrot and stick principle, intestinal bacteria growth could be stimulated by consuming a sweet dish. This would weaken the bacteria if an antibiotic against an intestinal infection is then administered,” explains Gerland. However, it is still too early for concrete recommendations. More research will be necessary.

Publication:

Elena Biselli, Severin Josef Schink, Ulrich Gerland:

Slower growth of Escherichia coli leads to longer survival in carbon starvation due to a decrease in the maintenance rate

Molecular Systems Biology, (2020) 16:e9478 – DOI: 10.15252/msb.20209478

https://www.embopress.org/doi/full/10.15252/msb.20209478

More information:

The research was funded by the German Research Foundation (DFG; SPP1617 and SFB / Transregio 174), a DFG research grant as part of the Graduate School of Quantitative Biosciences Munich (QBM) and a research grant from the European Molecular Biology Organization (EMBO). The investigations were done at the Chair for Physics of Complex Biosystems and in the TUM Research Center for Industrial Biotechnology.

Physiology of survival

https://www.tum.de/nc/en/about-tum/news/press-releases/details/35582/

E. coli-metabolism

https://www.tum.de/nc/en/about-tum/news/press-releases/details/34372/

High resolution images:

https://mediatum.ub.tum.de/1554425

Contact:

Prof. Dr. Ulrich Gerland

Professorship Theory of Complex Biosystems

Technical University of Munich

James-Franck-Str. 1, 85748 Garching, Germany

Tel.: +49 89 289 12394 – E-mail: gerland@tum.de

Web: https://www.groups.ph.tum.de/qbio/

The Technical University of Munich (TUM) is one of Europe’s leading research universities, with around 600 professors, 43,000 students, and 10,000 academic and non-academic staff. Its focus areas are the engineering sciences, natural sciences, life sciences and medicine, combined with economic and social sciences. TUM acts as an entrepreneurial university that promotes talents and creates value for society. In that it profits from having strong partners in science and industry. It is represented worldwide with the TUM Asia campus in Singapore as well as offices in Beijing, Brussels, Cairo, Mumbai, San Francisco, and São Paulo. Nobel Prize winners and inventors such as Rudolf Diesel, Carl von Linde, and Rudolf Mößbauer have done research at TUM. In 2006, 2012, and 2019 it won recognition as a German "Excellence University." In international rankings, TUM regularly places among the best universities in Germany. www.tum.de

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